Wednesday, June 10, 2009

Single Cell Genomics: New PLoS ONE article evaluated at Faculty of 1000

I recently handled an interesting article at PLoS ONE [Assembling the marine metagenome, one cell at a time. Woyke T, et al., PLoS ONE 2009 4(4):e5299].
As for the other high ranking PLoS ONE articles, this article too was evaluated at the Faculty of 1000 Biology and was rated with a F1000 Factor of 6.0 (Must Read). A redacted version of the evaluation by Douglas Bartlett is here: 
'This work details the use of single cell genomics from two uncultured marine Flavobacteria to recruit considerable sequence data from the Global Ocean Survey (GOS) compared to genome sequences from cultured Flavobacteria isolates. The genome reconstruction and stringent quality control of the two SAGs (single amplified genomes) are well handled, considering contamination and chimeras associated with the amplification process. The analyses of the two SAGs indicate unique metabolic features such as hydrogen oxidation, proteorhodopsin photometabolism, and biopolymer hydrolysis. Also of interest is the genome streamlining in the ...' ..... Full evaluation of the article is available here (

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